Chinese investigators studied Keratin 17 (KRT17), a 48KDa type I intermediate filament, which is mainly expressed in epithelial basal cells. KRT17 has been shown to be overexpressed in many malignant tumors and play an important role in the occurrence and development of tumors. The team explored the role and underlying mechanism of KRT17 in non-small cell lung cancers. (NSCLC).

Study

The team examined KRT17 expression and its correlations with clinicopathological factors in lung cancer tissue by immunohistochemistry. The prognosis value of KRT17 in NSCLCs was retrieved from the Cancer Genome Atlas (TCGA) online databases, reports Dovepress.

The expression level of KRT17 was increased or decreased by KRT17 gene transfection or small RNA interference in lung cancer cells, respectively. Furthermore, the proliferation and invasiveness of lung cancer cells were determined by cell proliferation and invasion assays, respectively. Finally, expression levels of proteins related to Wnt signaling pathways and epithelial mesenchymal transition (EMT) were detected by western blot.

Results

KRT17 expression were much higher in NSCLCs than normal lung tissues. High expression of KRT17 predicted poor prognosis of patients with NSCLCs, especially lung adenocarcinomas, and was correlated with poor differentiation and lymphatic metastasis.

Overexpression of KRT17 enhanced, while KRT17 knockdown inhibited the proliferation and invasiveness of lung cancer cells. Overexpression of KRT17 up-regulated β-catenin activityand levels of Wnt target genes, such as cyclin D1, cMyc and MMP7. Moreover, KRT17 promoted EMT by up-regulating Vimentin, MMP-9, and Snail expression and down-regulating E-cadherin expression.

Conclusion

Overexpression of KRT17 is common in NSCLCs and indicates poor prognosis. Overexpression of KRT17 enhances the proliferation and invasiveness of NSCLC cells by activating the Wnt signaling pathway and EMT process. KRI17 is a potential indicator (biomarker) of NSCLC progression and poor survival.

Lead Research/Investigators

Zhao Wang, Department of Pathology, The First Hospital and College of Basic Medical Sciences of China Medical University, Shenyang 110001, People’s Republic of China

Mai-Qing Yang, Department of Pathology, The First Hospital and College of Basic Medical Sciences of China Medical University, Shenyang 110001, People’s Republic of China

Lei Lei, Department of Pathology, The First Hospital and College of Basic Medical Sciences of China Medical University, Shenyang 110001, People’s Republic of China

Liang-Ru Fei, Department of Pathology, The First Hospital and College of Basic Medical Sciences of China Medical University, Shenyang 110001, People’s Republic of China

Yi-Wen Zheng, Department of Pathology, The First Hospital and College of Basic Medical Sciences of China Medical University, Shenyang 110001, People’s Republic of China

Wen-Jing Huang, Department of Pathology, The First Hospital and College of Basic Medical Sciences of China Medical University, Shenyang 110001, People’s Republic of China

Zhi-Han Li, Department of Pathology, The First Hospital and College of Basic Medical Sciences of China Medical University, Shenyang 110001, People’s Republic of China

Chen-Chen Liu, Department of Pathology, The First Hospital and College of Basic Medical Sciences of China Medical University, Shenyang 110001, People’s Republic of China

Hong-Tao Xu Department of Pathology, The First Hospital and College of Basic Medical Sciences of China Medical University, Shenyang 110001, People’s Republic of China

Source: Dovepress

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